Western blot All lanes: YWHAH antibody at 2µg/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue All lanes: YWHAH antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA02174A0Rb at dilution of 1:20
IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supplier Page from CUSABIO Technology LLC for YWHAH Antibody